Tryptic peptide screening for primary immunodeficiency disease by LC/MS-MS

Estimation of Nifedipine in Human Plasma by Lcms/ms

Nifedipine and NifedipineD6, (IS) were extracted from the plasma by precipitation method and then separated on a Reverse phase chromatography using the mobile phase mixture of ammonium acetate and methanol at a flow rate of 1.0 ml/min. The analytes were detected in API 4000 Mass spectrometer in the positive atmospheric pressure chemical Ionization (APCI) mode with multiple reactions monitoring ...

Development of Screening for Primary Immunodeficiency

Primary immunodeficiencies are inherited disorders of the immune system, with an estimated prevalence of 1:500 in the USA. Yet, a majority of the patients are still undiagnosed. Most patients with a diagnosis come in contact with the healthcare due to complications caused by the immunological defects. In many cases, it is crucial that the patients receive treatment before their health is seriou...

High through put analysis of MALDI time-of-flight MS/MS and LCMS/MS data

Lymphocyte Galactocerebrosidase Activity by LC-MS/MS for Post-Newborn Screening Evaluation of Krabbe Disease.

BACKGROUND Deficiency of the lysosomal enzyme galactosylcerebrosidase (GALC) causes Krabbe disease. Newborn screening for Krabbe disease is ongoing, but improved methods for follow-up analysis of screen-positive babies are needed to better advise families and to optimize treatment. We report a new assay for the enzymatic activity of GALC in lymphocytes. METHODS T lymphocytes were isolated fro...

A Comparison of Animal Hemoglobins by Tryptic Peptide Pattern Analysis.

8 Volkin, E., in Fourth International Congress of Biochemistry (New York: Pergamon Press, 1959), vol. 7, p. 212. 1 Volkin, E., L. Astrachan, and J. L. Countryman, Virology, 6, 545 (1958). '0 Astrachan, L., and E. Volkin, Biochim. et Biophys. Acta, 29, 536 (1958). 11 Countryman, J. L., and E. Volkin, J. Bacteriol., 78, 41 (1959). 12 Volkin, E., and L. Astrachan, in The Chemical Basis of Heredity...